Autonomic Nervous System: Stress Responses
  • Human
  • Grade 6+

Autonomic Nervous System: Stress Responses

How does your body respond to stress? In this experiment, we explore how the autonomic nervous system regulates heart rate in “stressful” situations—like submerging your hand in ice-cold water!

About the Experiment

What Will You Learn?

What Will You Learn?

  • How the autonomic nervous system (sympathetic and parasympathetic) regulates heart rate.
  • How to record and analyze EKG signals (Heart) using a Human SpikerBox.
  • How cold-water immersion can induce both psychological and physiological responses.
Background

Background

Our nervous system isn’t just in our heads—it runs throughout the entire body. This extensive network allows the brain to coordinate far-flung bodily functions. Think of times when you’ve felt anxious or stressed: you may experience a racing heart, sweating, and that “butterflies in your stomach” feeling. The body prepares you for action! Now think of how you feel after a huge holiday meal—tired and relaxed, almost lulled into a restful state. Your body and brain interact continuously, modulating responses to keep everything running smoothly. In this experiment, we’ll create brief, safe stressors to observe how the body’s autonomic nervous system responds. We’ll use an ice-water bath (the "Ice Water Test"), to determine if this is a "fight or flight" or a "rest and digest" response.

Experiment

  1. Ice-Water Hand Submersion Experiment

    Ice-Water Hand Submersion Experiment

    Safety first: skip this test if you have a heart condition, high blood pressure, Raynaud’s or a history of fainting. Keep your hand in the ice water for up to 1–2 minutes, or take it out sooner if it hurts.

    Materials:

    • A large container (bucket/bowl)
    • Ice cubes + cold water (roughly a 3:1 ice-to-water ratio)
    • Towel for drying off

    Setup:

    • Fill the container 3/4 full with ice, then add cold water until the mixture is near 0°C (32°F).
    • Place EKG patches on each upper forearm near the elbow. Why near the elbow? You need to keep the electrodes dry!
    • Connect alligator clips from the SpikerBox: red leads to the upper forearm patches, black lead to a ground patch on the back of the hand that stays out of the water.
    • Launch your recording software (SpikeRecorder), verify the SpikerBox is recognized, and note your resting heart rate.

    Procedure:

    • Record baseline heart rate. To see if the heart rate changes, we need to know the "before ice" rate.
    • Submerge your hand. Carefully place your hand in the ice water (keeping the electrode patches dry above the forearm).
    • Record heart rate. Keep recording the whole time your hand is in the water—up to 1–2 minutes, or take it out sooner if it hurts. Then remove your hand and immediately note your heart rate.
    • Rest and repeat. Warm your hand back up, let your heart rate settle, and then repeat 2–3 times, logging the data to get an average.
Results & Analysis

Results & Analysis

How does your heart rate change? Was it dramatic? Does it vary between trials or individuals? Discuss how these findings align with either sympathetic vs. parasympathetic control.

Draw the pathway from cold and pain receptors in the skin of your hand, up your spinal cord to your brainstem, and back out along the autonomic nerves to your heart. Label where your EKG electrodes recorded.

NGSS alignment

Performance expectations

  • MS-LS1-3 Use argument supported by evidence for how the body is a system of interacting subsystems composed of groups of cells.

    Nervous and circulatory subsystems interact: a cold stressor changes the heart's rate.

  • MS-LS1-8 Gather and synthesize information that sensory receptors respond to stimuli by sending messages to the brain for immediate behavior or storage as memories.

    Cold on the skin is sensed and sent to the brain, which changes the heart rate.

  • HS-LS1-2 Develop and use a model to illustrate the hierarchical organization of interacting systems that provide specific functions within multicellular organisms.

    Draw the pathway from cold and pain receptors in the skin to the brainstem and back out to the heart: the autonomic nervous system linking brain and heart.

  • HS-LS1-3 Plan and conduct an investigation to provide evidence that feedback mechanisms maintain homeostasis.

    Measure heart rate before, during and after an ice-water dip and watch it settle once the cold ends; baroreflex feedback helps limit the change, though this lab does not measure it directly.

Science and engineering practices

  • 2 Developing and Using Models

    Draw the pathway from cold and pain receptors in your hand to your brainstem and back out to your heart.

  • 3 Planning and Carrying Out Investigations

    Record EKG before, during and after an ice-water hand dip, two or three trials.

  • 4 Analyzing and Interpreting Data

    Compare heart rates across trials and people.

  • 5 Using Mathematics and Computational Thinking

    Average the heart rate over repeated trials.

  • 6 Constructing Explanations and Designing Solutions

    Decide from your data whether the response fits sympathetic or parasympathetic control.

Crosscutting concepts

  • Cause and Effect

    The cold stressor changes heart rate.

  • Stability and Change

    Heart rate settles back after the stressor ends.

Disciplinary core ideas

  • LS1.A Structure and Function

    Feedback mechanisms keep internal conditions within limits.

  • LS1.D Information Processing

    Sensory input from the skin drives an automatic response.

Backyard Brains mapping using the book's method. Not in the book.