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Experiment: View Your Antagonistic Muscles

Now that you've completed the "Getting Started with the Muscle Spikerbox" experiment, you've learned about muscle physiology through viewing electromyograms (EMGs) of your own muscles. More specifically, you've observed the electrical impulses that muscles fibers create to cause a contraction of a muscle. In this experiment, we will take a wider perspective of the muscles. Here, you will play the protagonist in the epic adventure of learning about agonist and antagonist muscles and how we rely on them for balance, posture and smooth, controlled movements. The 2-Channel Muscle SpikerBox provides you the ability to learn on how muscles work together by allowing you to observe the electrical activity of two muscles at once!

Time 30 Minutes
Difficulty Beginner

What will you learn?

In this experiment, you can learn about the relationships between different pairs of muscles of your body. This will give you an understanding of the difference between agonist muscles (cause movement by contracting) and antagonist muscles (opposes a specific movement) and how the two combined allow us to perform all sorts of amazing motions with precision and balance. You will also learn the difference between flexor and extensor muscles.

Prerequisite Labs


As the previous experiment explained, when you decide to make a movement your upper motor neurons from the motor cortex of your brain send action potentials (spikes!) traveling down your corticospinal tract (spinal cord) where they synapse with lower motor neurons which continue to propagate the signal to the designated muscle fibers. There, each single motor neuron and the muscle fibers they innervate create a "motor unit". At this neuromuscular junction, a neurotransmitter called acetylcholine is released which leads to generation of an action potential in the muscle fiber. Upon receiving this excitatory signal, the muscles fibers contract. Movement!

Your body uses this cascade of electrical and chemical signalling to control motion, but it's important to point out that each movement doesn't rely on just one muscle, but often many! There are over 600 skeletal muscles in the human body! Most of these muscles function in "antagonistic pairs", which means that when one muscles contracts (shortens), the other in the pair relaxes with careful control to allow elongation (stretching). Coordinating muscles together in this way gives us the ability to move gracefully, for example touching the tip your nose without smacking yourself in the face. Also, to make precise movements, such as threading a needle.

Let's talk more about these "antagonistic pairs". A great example pair is your biceps brachii and triceps brachii.

Before we go any further, we need to first review a few anatomical terms of motion. "Flexion" is a bending movement where the angle between two parts decreases. Contracting your biceps exhibits flexion, i.e. it brings your forearm closer to your upper arm and decreasing the angle between the two. So, your biceps is described as a "flexor" muscle. In the illustration below, the image on the right shows the biceps flexing. The opposing muscle of a flexor is called the "extensor" muscle. Your triceps is an extensor. When you contract your triceps your arm straightens and the angle between the forearm and the upper arm increases. You may have already guessed but this is called "extension" and you can see that in the left illustration below. These designations are intrinsic, meaning they are an unchangeable property of the muscle. This means that contracting a flexor muscle will always exhibits flexion and never extension (and vis versa for contracting extensor muscles).

Okay, so now that we have our terms of motion established we can discuss these antagonistic pairs properly! The two muscles in an antagonistic pair are in opposition. That is, if one extends a limb during its contraction, the other will return the limb to its original position when flexed. In each pair, depending on the movement, one muscle plays the role of the "agonist" and the other muscle plays the role of "antagonist". The agonist is a muscle that contracts to cause the movement. The antagonist is an opposing muscle that relaxes relatively to stretch. These two roles, agonist and antagonist, can be exchanged back and forth. To visualize this, let's jump back to our biceps and triceps example. Image waving at your best friend: when your hand is moving away from you, your triceps is an agonist, contracting to extend your arm. Your biceps is an antagonist, relaxing to allow elongation while possibly contracting ever-so-lightly to control the speed of that moving forearm.

When your hand is moving back in during your waving motion your biceps is an agonist, flexing your arm towards you. In this case, your triceps is an antagonist and must relax to stretch to allow the movement. So you can see that unlike the intrinsic designations of the flexors and extensors, the two roles of antagonistic pairs are dependent on the motion. If the motion is reversed, the agonist and the antagonist switch roles.

It's not always this simple though! In some movements, such as a push-up, the agonist doesn't change with the direction of motion. During push-ups the triceps is the agonist whether you're lowering your body towards the ground or pushing up. This is because the applied load from gravity. With a constant force always pulling you down, you're triceps must remain in contraction to hold you up, whether you're moving up or down. Now, drop and give me 20!

Taking it one step further, it's important to realize that even when you are not moving you have muscles constantly working at your service to maintain posture. Yes, even when you are slouching in your La-Z-Boy your brain is commanding muscles to contract! So the key point is that when you think to make any movement or even just to stand still, our brain (specifically the motor cortex) is sending action potentials (spikes!) along our nerves to not just one but multiple muscles to coordinate the movement with control or simply just to stay balanced and upright.



Now let's put that 2-Channel Muscle SpikerBox to use by investigating our favorite antagonistic pair.

  1. Place two of your sticker electrode patches on your biceps and connect the 2 red alligator clips from channel 1 (the right side of the device) to the metal nub of the electrodes. Then place two electrode stickers on your triceps and connect the channel 2 red cables to them. Finally, place one sticker electrode on the back of your hand and connect both black alligator clips (from ch1 & ch2) to this electrode.
  2. Connect a stereo speaker to one of the 2-Ch Muscle SpikerBox's audio jacks. Connect (with the blue cable) your computer's line-in (a.k.a. microphone) to the other audio jack. Next, open the SpikeRecorder software. You will have to click the "Config" button in the top left of the SpikeRecorder to turn on both left and right inputs.

    Alternatively, you can connect the second audio jack to your smartphone (with the green cable) and open the Backyard Brains app. Free for download for: Android or iPhone. Note that both signals will be displayed together with the current app.

  3. Turn both EMG channels on by rotating each thumbwheel until you hear activity during contraction of your biceps and your triceps. Look at the app and make sure you can see your signals! If the signals are clipping (hitting the tops and bottoms of the window), lower the gain by turning the thumbwheel down.
  4. Now let the experimentation begin!

    A) Let's start with waving. Can you get someone to wave back at you? Which muscles do you think you are using to perform this action? To investigate your hypothesis, create an experiment by changing the placement of your sticker electrodes until you observe an EMG full of action potentials. Remember be a good scientist by documenting your method and collecting data!

    Pump up your gestural greeting with extra muscular effort so you can visualize/hear the EMG better. Can you use your two EMGs to verify which muscle is the agonist when you flex your arm? How about when you extend your arm?

    B) The antagonist muscle might show spiking as well, why do you think? Try to explore your arm motions until you can completely relax the antagonist (no spikes).

  5. Let's go a step further, do a push-up! (If the floor is dirty or you're just not a fan of push-ups, you can also do a push-up on the edge of a sturdy table by leaning into it.) Can you verify with your EMGs that the triceps is the agonist whether you are going up or down?

    Here are our results (biceps EMG is in orange above, triceps EMG is in yellow below):

    This screenshot above shows the two EMGs during the pushing up phase of a push-up. The biceps look like the relaxed antagonist and those triceps are definitely firing more spikes! The screen capture above shows the two EMGs during the lowering phase of a push-up. We can see that even though the direction of motion has reversed, the triceps are still bearing the load and playing the role of the agonist.
  6. Further experimentation suggestions:

    Can you think of other antagonistic pairs in your body? Test out your hypothesis by recording EMGs from those two muscles to see if you are right. Remember, one should show lots of spikes (agonist) while the other shows few or none at all (antagonist) and vis versa. Another fun exploration would be to try and find two muscles that display action potentials in their EMGs even when you are standing or sitting still. The 2-Channel Muscle SpikerBox can also let you compare your muscles to your friends'. Observe your EMGs while arm wrestling!

    Or try a hands-free arm wrestling match. Winner goes to whoever can keep their spikes up longer!

    Take advantage of these two channels and let us know your experimental results! Email us your process and findings at!


    If you have our original 2 channel Muscle SpikerBox with 6 seperate leads (not 2 orange cable), the colors of the alligator clips are different. The Red and Black clips should go around the muscle of interest, while the white is used as the ground electrode.